I can't tab through controls, if they are in a layout container such as a VBox (I also tested with StackPane) and one container is invisible.

There was a very similar issue here:
RT-18447


Test case:

import javafx.application.Application;
import javafx.scene.Scene;
import javafx.scene.control.TextField;
import javafx.scene.layout.VBox;
import javafx.stage.Stage;
 
 
public class TestApp extends Application {
 
 
    public static void main(String[] args) throws Exception {
        launch();
    }
 
    public void start(final Stage stage) throws Exception {
 
        VBox root = new VBox();
 
        VBox vBox = new VBox();
        TextField textField1 = new TextField();
        vBox.getChildren().add(textField1);
 
        VBox vBox2 = new VBox();
        TextField textField2 = new TextField();
        vBox2.getChildren().add(textField2);
        vBox2.setVisible(false);
 
        TextField textField3 = new TextField();
 
        root.getChildren().addAll(vBox, vBox2, textField3);
 
 
        Scene scene = new Scene(root);
        stage.setScene(scene);
        stage.show();
    }
}